Signalling

Part:BBa_K3267000:Design

Designed by: Yuma Kanazawa   Group: iGEM19_NYU_Shanghai   (2019-10-15)


Bioelectric Relay Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 49


Design Notes

Added ssrA tag after LuxI coding sequence.


Source

C-terminal peptide that mediates degradation in bacteria through the ClpXP and ClpAP proteases (McGinness et al., 2006)

References